WAY-333945，689273-45-8，10mM in

Competent Cell Preparation

DMSO (the DMSO needs to be stored at -20°C o/n before use).  8. Aliquote the cell to 50 to 500ul for transformation or store at -70°C.  *Note: The E. coli cells prepared this way are normally 100 to 1000 times more efficient

重组DNA的分离、克隆与测序实验手册

9.0 4 ml 1 M (NH4)2SO4, pH 9.0 25 mM MgCl2, pH 7.0 1 ml 1 M MgCl2, pH 7.0 5% DMSO 2 ml DMSO 17 ml ddH2O 40 ml The 5X Taq reaction buffer will be added separately with the A, C, G, and T nucleotide mixes

In Situ HYBRIDIZATION TO TISSUE SECTIONS

  In Situ HYBRIDIZATION TO TISSUE SECTIONS (Kornberg et al., Cell 40:45, 1985)) PARAFFIN SECTIONS Preparation of embryos (¾10 hr) for paraffin sections Wash embryos off collection plates onto a nylon screen with 0.4% NaCl