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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
WAY-615145
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
536736-95-5
- 规格:
W424584-1ml
英文名:WAY-615145
纯度或规格:10mM in DMSO
SPU:W424584
CAS:536736-95-5
存储温度:-80℃储存
运输条件:特低温运输
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文献和实验the gel: dissolve 0.8g agarose in 100mL of 10mM NaPO4 pH 7.0 (5mL of 0.2M NaP04 pH 7.0 + 95mL "DEPC" H2 O). Make sure gel box and comb are RNase-free by treatment with 3% H2 O2 or commericial RNase removal product (e.g. RNase Zap from Ambion).
In Situ HYBRIDIZATION TO TISSUE SECTIONS
dechorionated embryos to either buffer A or B (depending on their age): Buffer A (0-2, 1-3 hr embryos) 2.5 ml 8% paraformaldehyde (fresh) 2.0 ml 1X PBS 0.5 ml DMSO - or - Buffer B (2-4, 3-5
Mutation Detection By Single-Strand Conformational Polymorphism (SSCP)
3.84ml 10% APS 256µl TEMED 25.6µl Pour sequencing gel format with appropriate sharkstooth comb. Gel will polymerize in about 1 hour. Loading Buffer 95% formamide 10mM
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