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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
WAY-345057
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
312591-60-9
- 规格:
W423216-1ml
英文名:WAY-345057
纯度或规格:10mM in DMSO
SPU:W423216
CAS:312591-60-9
存储温度:-80℃储存
运输条件:特低温运输
查看阿拉丁官网此产品相关对应页面:
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文献和实验solution is distributed into 0.5 ml colored microcentrifuge tubes (blue for A, green for C, purple for G, and yellow for T) in 60 or 120 ul aliquots (60 for A and C/120 for G and T). The simplest way to distribute the 60 ul aliquots is 2 x 30 ul using
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
The easiest way to heat these solution is to submerge large, double zipper plastic bags in water bath. The temperature used varies for different probes and samples. Make sure you know what tempt to use. 60 C is standard for homologous DNA/DNA/RNA hybs
In Situ HYBRIDIZATION TO TISSUE SECTIONS
DNase (1U/ml) to the 24ml reaction mixture and incubate 30' @ 37 degrees C. Stop the reaction: +60ml 10mM EDTA, 10mM Tris-HCl (pH 7.4), 0.2% SDS +1.67ml 5M NaCl +1.0ml 1M DTT Incubate 10-15' on ice Hydrolyze the probe (ideal
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