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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
WAY-332076
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
1015865-34-5
- 规格:
W420353-1ml
英文名:WAY-332076
纯度或规格:10mM in DMSO
SPU:W420353
CAS:1015865-34-5
存储温度:-80℃储存
运输条件:特低温运输
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
gr/l Difco bacto agar. Hypoclorite solution: for 50 ml: 4ml Na Hypoclorite 15% 255l Tween-20 water to 50ml cDNA PROBES mRNA 1-5ug H2O 50ul oligoDt (10mg/ml) 2ul heat 90C 2'', ice 5xRT buffer 20 10mM
9.0 4 ml 1 M (NH4)2SO4, pH 9.0 25 mM MgCl2, pH 7.0 1 ml 1 M MgCl2, pH 7.0 5% DMSO 2 ml DMSO 17 ml ddH2O 40 ml The 5X Taq reaction buffer will be added separately with the A, C, G, and T nucleotide mixes
This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when the nuclei are salt extracted and the resulting proteins AMSO4 precipitated. The high
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