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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
N-Nitrosodiethylamine
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
55-18-5
- 规格:
N424700-1ml
英文名:N-Nitrosodiethylamine
纯度或规格:10mM in DMSO
SPU:N424700
CAS:55-18-5
存储温度:-80℃储存
运输条件:特低温运输
查看阿拉丁官网此产品相关对应页面:
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文献和实验and autoclave to sterilize. 2. TB solution: *10mM PIPES *15mM CaCl 2 (1.66g/L) *250mM KCl(18.6g/L) *Dissolve in nanopure water and adjust pH to 6.7 with KOH or HCl and then add MnCl 2 to 55mM
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
, gv3101 - 25GEN 2. Transfer O/N culture to 200ml YEP in a sterile 500ml flask and shake at 250rpm until the OD is 0.3 (4-5hrs) 3. Spin in sterile 50ml screw cap tubes 4C 5krpm 10‚. Check to make sure cells are pelleted, if not repeat at higher
in a thermocycler. Heat sample to 95°C, then add 0.5 -1 ul of enzyme (Taq, Tli, Pfu etc.). Add a few drops of mineral oil.3.Start the PCR cycles according the following schemes:(1) denaturation - 94 ° C, 30-90 sec.(2) annealing - 55 °C (or -5° Tm), 0.5-2 min
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