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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
LB-100
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
1026680-07-8
- 规格:
L420390-1ml
英文名:LB-100
纯度或规格:10mM in Water
SPU:L420390
CAS:1026680-07-8
存储温度:-80℃储存
运输条件:特低温运输
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文献和实验Purification of GST fusion proteins in E.coli GST融合蛋白纯化,方法一
Making GST fusion proteins:(07/19/03)ver.1 Grow up 5ml LB with Amp o/n. Add to 45ml LB with Amp 37o shake 2.5 - 3 hrs,till OD600 0.4-0.8 Put bottles in room temperature water for 10 min to cool down. Add 100μl 0.2M IPTG to 0.4mM final Shake 30
Transformation of E. coli by Electroporation [Stanford University]
Preparation of electroporation cells 1. Prepare an overnight of NM522 in minimal medium. 2. Inoculate 1L LB with 10ml (1/100th vol) of the overnight and grow to A600 = 0.5- 1.0. 3. Pellet cells 5krpm, 15' @ 4℃. 4. Wash cells in 1L ice-cold water
Bacterial Expression of GST-fusion Proteins
1. Grow cells in 5ml (or more) of LB-Amp overnight for a starter culture. 2. Grow larger culture (100x volume of starter culture) using the overnight culture as a seeding culture. The culture is grown in LB-Amp medium. Aerate
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