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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
LY2109761
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
700874-71-1
- 规格:
L408237-1ml
英文名:LY2109761
纯度或规格:Moligand™, 2mM in DMSO
SPU:L408237
CAS:700874-71-1
存储温度:-80℃储存
运输条件:特低温运输
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文献和实验Troubleshooting for PCR and multiplex PCR
, but keep MgCl2 concentration at 1.5-2mM Increase MgCl2 concentration up to 3-4.5 mM but keep dNTP concentration constant. Add adjuvants. Best, use BSA (0.1 to 0.8 µg/µL final concentration). You can also try 5% (v/v, final concentration) DMSO or glycerol
unspecific products. What can I do?Decrease annealing timeIncrease annealing temperatureDecrease extension timeDecrease extension temperature to 62-68º CIncrease KCl (buffer) concentration to 1.2x-2x, but keep MgCl2 concentration at 1.5-2mM.Increase MgCl
Cell Culture Media and Solutions
the tubes at -80 degrees C for up to one year. 700 ml RPMI-1640 with 2mM L-Glutamine 200 ml fetal bovine serum (FBS) 100 ml dimethyl sulfoxide (DMSO, Sigma) -------- 1000 ml total volume Note: Filter sterilize media and FBS
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