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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
Iminostilbene
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
256-96-2
- 规格:
I422898-1ml
英文名:Iminostilbene
纯度或规格:10mM in DMSO
SPU:I422898
CAS:256-96-2
存储温度:-80℃储存
运输条件:特低温运输
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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
the denaturation time is 1 min at 94℃: it is possible, for short template sequences, to reduce this to 30 sec or less. Increase in denaturation temperature and decrease in time may also work: Innis and Gelfand (1990) recommend 96℃ for 15 sec.Annealing Temperature
Mutation Detection By Single-Strand Conformational Polymorphism (SSCP)
3.84ml 10% APS 256µl TEMED 25.6µl Pour sequencing gel format with appropriate sharkstooth comb. Gel will polymerize in about 1 hour. Loading Buffer 95% formamide 10mM
9.0 4 ml 1 M (NH4)2SO4, pH 9.0 25 mM MgCl2, pH 7.0 1 ml 1 M MgCl2, pH 7.0 5% DMSO 2 ml DMSO 17 ml ddH2O 40 ml The 5X Taq reaction buffer will be added separately with the A, C, G, and T nucleotide mixes
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