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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
Iminostilbene
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
256-96-2
- 规格:
I422898-1ml
英文名:Iminostilbene
纯度或规格:10mM in DMSO
SPU:I422898
CAS:256-96-2
存储温度:-80℃储存
运输条件:特低温运输
查看阿拉丁官网此产品相关对应页面:
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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
the denaturation time is 1 min at 94℃: it is possible, for short template sequences, to reduce this to 30 sec or less. Increase in denaturation temperature and decrease in time may also work: Innis and Gelfand (1990) recommend 96℃ for 15 sec.Annealing Temperature
Mutation Detection By Single-Strand Conformational Polymorphism (SSCP)
3.84ml 10% APS 256µl TEMED 25.6µl Pour sequencing gel format with appropriate sharkstooth comb. Gel will polymerize in about 1 hour. Loading Buffer 95% formamide 10mM
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
411400 (Boehringer). For 10mM stock, weigh out 1mg (light sensitive!), add 27ul DMSO to solubilize, then add 12ul 3M NaOAc, pH5, mix, then 275ul H2O, Don''t make up large amountrs as this is not very stable, even at -80C. Diluted luciferin solution
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