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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
避光;-20°C储存;充氩
- 英文名:
DSPE PEG Fluorescein, DSPE-PEG-FITC
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
D163612-50mg/D163612-250mg
| 规格: | D163612-50mg | 产品价格: | ¥989.9 |
|---|---|---|---|
| 规格: | D163612-250mg | 产品价格: | ¥3148.9 |
1,2-Distearoyl-sn-Glycero-3-Phosphoethanolamine (DSPE) conjμgated and fluorescein labeled Polyethylene Glycol, DSPE PEG Fluorescein is a phospholipid PEG conjμgate which has both hydrophilicity and hydrophobility. Pegylated phospholipids are excellent liposome formation materials that can be used for drμg delivery, gene transfection and vaccine delivery as well. Pegylation of phospholipids significantly improves the blood circulation time and stability for encapsulated drμgs. These materials can also be used for targeted drμg delivery by modifying their surfaces with targeting ligands such as antibodies, peptides. Fluorescein labeled DSPE PEG products emitted green fluorescence and can be easily detected with regular fluorescent instrucments.
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文献和实验Angiogenic Vessel-Targeting DDS by Liposomalized Oligopeptides
of PEGylated liposomes, we synthesized a novel lipid derivative of the peptide, distearoylphosphatidylethanolamine–polyethyleneglycol–APRPG (DSPE–PEG–APRPG). The lipid derivative of APRPG peptide is expected to be readily incorporated into liposomal membrane
。 测定RB200荧光抗体的克分子比值公式如下: 按图3-4测定法更为简便,即先用276nm波长测得蛋白质的OD值,再用493波长测得FITC的OD值,将这两个OD值在图3-14上连成一直线,直线与各纵线交叉处,即可查出标记抗体的以下数值:FITc μg/ml ,F/P 的μg/mg,F/P的克分子比值,蛋白mg/ml等。 四、荧光抗体的保存 以0~4℃或-20℃低温保存,防止抗体活性降低和蛋白变性。最好加入浓度为1:5000~10000的硫柳汞或1:1000~
6-sulfonic acid),2,2'-连氮基-双(3-乙基苯并噻吡咯呆-6磺酸)。6、96孔ELISA板。7、0.15mol/L,pH7.4 PBS:配其它液体用。8、包被缓冲液: 0.05mol/L,pH9.6 Na2CO3-NaHCO3缓冲液。9、洗涤液: 0.05%Tween 20-PBS。10、稀释液:4%PEG-洗涤液(用于稀释标准品和酶标抗体)。11、底物缓冲液:0.1mol/L,pH5.0柠檬酸-磷酸氢二钠缓冲液。12、3%H2O2。13、血清、枸缘酸盐或EDTA抗酸血浆、其它体液及细胞
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