- ￥205.90
- 阿拉丁
- 上海
- C424360
- 2026年04月21日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80℃储存
- 英文名：
Chloroquine Phosphate
- 库存：
现货
- 供应商：
上海阿拉丁生化科技股份有限公司
- CAS号：
50-63-5
- 规格：
C424360-1ml
英文名：Chloroquine Phosphate
纯度或规格：10mM in Water
SPU：C424360
CAS：50-63-5
存储温度：-80℃储存
运输条件：特低温运输
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文献和实验Transformation of E. coli by Electroporation [Stanford University]
Preparation of electroporation cells 1. Prepare an overnight of NM522 in minimal medium. 2. Inoculate 1L LB with 10ml (1/100th vol) of the overnight and grow to A600 = 0.5- 1.0. 3. Pellet cells 5krpm, 15' @ 4℃. 4. Wash cells in 1L ice-cold water
Bacterial Expression of GST-fusion Proteins
Beads can be stored at 4oC overnight and/or test an aliquot on a protein gel. 11. Elute the fusion protein from the sepharose 3x with 10mM glutathione, 50mM Tris pH8.0, 10mM DTT, and 0.5M NaCl. 12. Dialyze fusion protein against 50
for 5 minutes. DO NOT LET THE SAMPLES SIT FOR MORE THAN 5 MINUTES BEFORE PROCEEDING TO THE NEXT STEP!!!!!!!!!! Solution III: 200 ml-> 50 ml 7.5 M K-acetate, 23 ml Acetic Acid, and 127 ml water. Make at start of day and put in fridge or on ice so is cold
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