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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide derived from human MSH6 (AA 1-57)
- 亚型:
Rabbit IgG
- 保存条件:
-20°C储存,避免反复冻融;超低温运输
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 浓度:
见COA
- 规格:
Ab116112-100μl/Ab116112-1ml/Ab116112-50μl/Ab116112-10μl
| 规格: | Ab116112-100μl | 产品价格: | ¥2099.90 |
|---|---|---|---|
| 规格: | Ab116112-1ml | 产品价格: | ¥16999.90 |
| 规格: | Ab116112-50μl | 产品价格: | ¥1299.90 |
| 规格: | Ab116112-10μl | 产品价格: | ¥499.90 |
基本信息
产品名称:Recombinant MSH6 Antibody
规格或纯度:重组, ExactAb™, 已验证, 见COA
应用:WB, IF/ICC, IHC
特异性:MSH6
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原:A synthetic peptide derived from human MSH6 (AA 1-57)
阳性对照:WB: HeLa and C6 cell lysates. IHC: Human colon carcinoma tissue. IF/ICC: HeLa cells.
偶联:Unconjugated
产品属性
克隆类型:重组抗体
Format:Whole IgG
亚型:Rabbit IgG
轻链亚型:kappa
SDS-PAGE:150 kDa
纯化方法:Protein A purified
物理外观:Liquid
储存缓冲液:10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH7.4
防腐剂:0.02% Sodium azide
浓度:见COA
储存条件:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:兔(Rabbit)
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
偶联:Unconjugated
图片
Recombinant MSH6 Antibody (Ab116112) - Western Blot
All lanes: Recombinant MSH6 Antibody (Ab116112) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 153 kDa
Observed band size: 178 kDa
Exposure time: 3.2 s
Recombinant MSH6 Antibody (Ab116112) - Western Blot
All lanes: Recombinant MSH6 Antibody (Ab116112) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 153 kDa
Observed band size: 178 kDa
Exposure time: 2.3 s
Recombinant MSH6 Antibody (Ab116112) - IHC
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Recombinant MSH6 Antibody (Ab116112).
Recombinant MSH6 Antibody (Ab116112) - IF/ICC
Immunofluorescent analysis of MSH6 in HeLa cells, using the Recombinant MSH6 Antibody (Ab116112) at 1/50 dilution.
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文献和实验Antibody Validation by Western Blotting
must be thoroughly demonstrated using a complex biological sample, rather than a recombinant protein, prior to use in clinical translational research. In the simplest iteration, antibody specificity is determined by the presence of a single band in a complex
Production of Monoclonal Antibody by DNA Immunization with Electroporation
associated with problematic antigens: low abundance and protein instability and use of recombinant proteins that lack posttranslational modifications. This chapter describes the use of a DNA-based immunization protocol for the production of mAb
VERIFY overexpression lysate antigen standards (Lane 1 to 7) were fractionated on SDS-PAGE, and then immunoblotted with 8F1 (Upper panel)。 The recombinant protein expression levels within the lysates were analyzed with anti-DDK antibody (Lower panel
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