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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C储存
- 英文名:
Indirubin
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
479-41-4
- 规格:
I111267-20mg/I111267-10mg/I111267-5mg/I111267-50mg/I111267-100mg
| 规格: | I111267-20mg | 产品价格: | ¥292.9 |
|---|---|---|---|
| 规格: | I111267-10mg | 产品价格: | ¥226.9 |
| 规格: | I111267-5mg | 产品价格: | ¥142.9 |
| 规格: | I111267-50mg | 产品价格: | ¥584.9 |
| 规格: | I111267-100mg | 产品价格: | ¥934.9 |
Indirubin, also known as isoindigotin, is an inhibitor of cyclin-dependent kinases (CDK1/cyclin B (IC50 = 10μM), CDK2/cyclin A (IC50 = 2.2μM), CDK2/cyclin E (IC50 = 7.5μM), CDK4/cyclin D1 (IC50 = 12μM), CDK5/p35 (IC50 = 5.5μM)) and of GSK-3β (IC50 = 600nM). Studies investigating functional isoindigos show that they have the ability to disrupt cell growth and proliferation. The compound is a potent aryl hydrocarbon receptor ligand and is found in human urine and fetal bovine serum. Synthesized isoindigo-based oligothiophenes are used to study heterojunction solar cells. Shown to inhibit allergic contact dermatitis by regulating T helper mediated immune system in a DNCB-induced model. An inhibitor of GSK-3β and cyclin-dependent kinases.
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文献和实验≥95 ≥90 0 20 ≥90 ≥85 1 48
Advantages Cat. No. Taq DNA Polymerase •Sequencing•Safeguarding sample•In vitro transcription TOPO® Cloning(5 minutes) Topoisomerase TOPO TA Cloning® Kit •≥95
培养 24 - 48 h,细胞汇合度达到 80% - 95% 左右时收取上清,该上清液即可用于提取外泌体。 使用外泌体专用无血清培养基: 待细胞汇合度~50%时,移去原有完全培养基,添加新培养基进行培养,新培养基由 50% 外泌体专用无血清培养基 + 50% 完全培养基组成; 待细胞生长汇合度约为70-80%时,移去培养基; 使用1×PBS溶液将细胞润洗2-3次; 加入外泌体专用无血清培养基继续培养24 - 48 h,待细胞汇合度到 80% - 95% 时收取细胞培养上清液,该上清液即可用于外泌
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