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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C储存,充氩;低温运输
- 英文名:
Sodium Dodecylbenzenesulfonate(mixture) solution(SDBS)
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
25155-30-0
- 规格:
S196342-20ml/S196342-50ml
| 规格: | S196342-20ml | 产品价格: | ¥205.90 |
|---|---|---|---|
| 规格: | S196342-50ml | 产品价格: | ¥399.90 |
中文名:十二烷基苯磺酸钠(SDBS)
英文名:Sodium Dodecylbenzenesulfonate(mixture) solution(SDBS)
纯度:1000ug/ml in water
货号:S196342
Cas号:25155-30-0
存储温度:2-8°C储存,充氩
运输条件:低温运输
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文献和实验of a 10% gel 4 ml H2O 3.3ml 30% acrylamide/bisacrylamide (29:1 mix) 2.5 ml1.5M Tris(PH 8.8) 10% SDS 100 µl 10% ammonium persulfate 100 µl TEMED 8 µl 2. Stacking gel: 4 ml 2.7ml water 1 ml 0.67ml 30% acrylamide/bisacrylamide (29:1 mix) 0.ml 1.0M
7b. Pool the supernatants from step 6 into 500 ml bottles and add DNase-free RNase A and RNase T1 such that the final concentration of RNase A is 40 ug/ml and RNase T1 is 40 U/ml. Incubate in a 37degC water bath for 30 minutes. 8b. Add an equal
Polyclonal and Monoclonal Antibodies
% ( 1000X ) Protocol: 1) ADSORBTION OF ANTIGEN a) Dilute Ag to 10 ug/ml in PBS. b) Add 100 ul of Ag solution to each well. c) Leave O.N covered with saran wrap, at 4 C. 2) BLOCKING a) Wash unbound Ag by inverting
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