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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
Sodium carbonate
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
497-19-8
- 规格:
S424284-1ml
英文名:Sodium carbonate
纯度或规格:10mM in Water
SPU:S424284
CAS:497-19-8
存储温度:-80℃储存
运输条件:特低温运输
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文献和实验主要试剂 1. SOB solution: *0.5% yeast extract *2% tryptone *10mM NaCl(0.581g/L) *2.5mM KCl(0.191g/L) *10mM MgCl 2 *10mM MgSO 4 *Dissolve in nanopure water
Protocol for First-strand cDNA Synthesis
the mix at 70℃for 5 minutes and chill on ice.Add the following in the order indicated:5X reaction buffer 4µl,10mM 4 dNTP mix 2µl (1.0mM - final concentration),ribonuclease inhibitor 20u,deionized water (nuclease free) to 19µl.Incubate at 37℃for 5 minutes
In vitro RNA synthesis from plasmid-borne sequences
N.B: Gloves should be worn at all times during preparation of in vitro RNA. All solutions should be RNase-free i.e. made with DEPC water if home-made or bought specifically to use for RNA work.You will need:100mM DTT (Gibco-BRL)Ribonuclease
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