胶原酶 NB 1 来源于溶组织梭菌，9001-12-1，En

Description

This highly purified collagenase quality is used for isolation of sensitive cells and guarantees high yields and viability. In combination with Neutral Protease NB Collagenase NB is especially applicable to isolation of pancreatic islets of Langerhans.

Collagenase NB is recommended for applications which have to meet requirements like tissue engineering and transplantation into humans.

Specifications

Contains chromatographically highly purified class I and class II collagenase (1).

Largely free from clostripain, trypsin-like protease and neutral protease.

Vial contains not less than 2000 PZ U collagenase.

PZ activity (Wünsch): ≥ 3.000 U/mg

Endotoxin ≤ 10.0 EU**/mg (Ph. Eur.)

Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia, abnormal toxicity (general safety test; Ph. Eur.).

Manufactured according to EU GMP guidelines.

(Clostridiopeptidase A)

EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]

References and Definitions

*Unit definitions:Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

**Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO appoved reference standard endotoxin (RSE)].

References:

1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091

2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51