Recombinant Human Cathepsin D

Protein Folding Liquid Chromatography

and a new chromatographic cake. A protocol for fast renaturation with simultaneous purification of inclusion body protein of the recombinant human interferon-gamma to obtain purity ≥95% and high specific bioactivity in a single step and in 1 h is introduced.

Protein arginine methylation

with corresponding (for example NP40-1% buffer). Keep samples at 4°C during washing steps d) Add of SDS-loading buffer were added to each sample (optimal 40-60 μl). Heat samples at 95°C for 5 min and analyse by SDS-PAGE. Incubated for 1 h with H3 enhancer

Introduction of human 14-3-3 proteins

The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14