Recombinant MPXV M1R Protein，A

Cell-Free Protein Synthesis as a Promising Expression System for Recombinant Proteins

Cell-free protein synthesis (CFPS) has major advantages over traditional cell-based methods in the capability of high-throughput protein synthesis and special protein production. During recent decades, CFPS has become an alternative protein

Protein Syntheses in Cell Free Systems

equilibrated with 1X TBS-M buffer•Liquid nitrogen storage•Reaction mixture for protein synthesis,containing the following in a total volume of 50 µl 2Tris-HCl,pH 7.5 1.5 µmMg acetate 0.15 - 0.20 µmKCL 4.0 - 5.0 µm-Mercaptoethanol0.25 µmATP 0.05 µmGTP 0.005 µ

An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources

results in protein productivity of about 50 μg per mL of the translation reaction mixture. This is the highest protein productivity yet noted among commercialized cell-free protein synthesis systems based on animal extracts.