高纯超细碳氮化钛粉体，12654-86-3，≥98%, pa

PKH 不适合染外泌体？看看文献怎么说

到大量纳米颗粒，可能是胶束和聚集体，颗粒分布在80-400 nm，与外泌体所在的粒径区间（30-150 nm）存在交集，这与之前报告的结果一致[2, 3]。PKH聚集而成的纳米颗粒自身的荧光干扰不容忽视，Lai 等人[4]研究表明用 PKH67 染色（没有 EV）甚至比 PKH67 染色的 EV 具有更强的荧光信号，这些同样具有荧光的纳米颗粒会“伪装”成外泌体，影响标记阳性率、外泌体粒径分布和细胞摄取结果。 Fig.1 NTA视频观察到大量聚集的PKH颗粒   2. 弥散造成的假阳

Tracking individual chromosomes with integrated arrays of Lacop sites and GFP-Laci repressor

-lapse series are as follows: 5-11 optical z slices taken every 1 to 4 minutes, z sections are 200-400 nm in depth and the exposure time is ~50ms. Using these settings up to 300 stacks of 5 sections each (1500 frames) at 1 minute intervals can be captured

Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy

is also frequently useful: M (Da) = 825 V (nm3 ). What we really want is a physically intuitive parameter for the size of the protein. If we assume the protein has the simplest shape, a sphere, we can calculate its radius. We will refer to this as R min