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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 库存:
50
- 供应商:
北京沃比森科技有限公司
- CAS号:
1045020-74-3
- 规格:
0.5mg/1mg
| 规格: | 0.5mg | 产品价格: | ¥5168.0 |
|---|---|---|---|
| 规格: | 1mg | 产品价格: | ¥9898.0 |
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文献和实验Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction
, with more than 70% recovery. The LC-fractionated proteins are further separated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE). Protein bands are excised, in-gel digested with trypsin, and then analyzed
sodium phosphate , pH 7 alternatively use 1 M acetic acid for washing ( 3 - 4 CV) alternatively use 100 mM PBS , pH 7.4 with Proclin 0.01
Site-directed hydroxy radical mapping of nucleosome positions in vitro
on ice (see note 13); Freshly prepare reagent solution by degassing 3 small sidearm flasks, each containing 50ml water. Add 37.5mg of ferrous ammonium sulphate, 200mg of ascorbic acid and 333µl of hydrogen peroxide to separate flasks. Swirl
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