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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
- 保质期:
1 year
- 英文名:
CTNNB1, N-His, recombinant protein
- 库存:
999
- 供应商:
ProteoGenix
- 规格:
50ug/100ug
| 规格: | 50ug | 产品价格: | ¥1856.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥2456.0 |
| Species | Homo sapiens (Human) |
| Isotype | nan |
| Expression system | Prokaryotic expression |
| Buffer | PBS, pH7.5 |
| Size | 100 ug |
| Aliases / Synonyms | nan |
| Delivery condition | Dry Ice |
| Storage condition | 4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection) |
| Kit content | nan |
| Tag | N-terminal His Tag |
| Fragment | Met1-Leu781 |
| Host Species | Escherichia coli (E.coli) |
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文献和实验蛋白质原料的氨基酸,由 ATP活化变成氨酸基 tRNA的形式 tRNA既具有活化氨基酸载体的作用,同时也具有解读遗传信息受体的功能。氨酰基 tRNA和 mRNA结合在核蛋白体上形成多核蛋白体,边进行遗传密码的翻译,边进行蛋白质的合成。蛋白质的生物合成从 N- 末端开始朝向 C末端进行。 mRNA上的密码是从 5′末端朝着 C末端翻译。从而, mRNA的 N′末端有相当于肽链的 N末端的起始点存在,在这里开始肽链的合成,随之肽链的延长,最后到 C末端蛋白质合成终结。已合成的蛋白质可从核蛋白体上游
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
, and usually final protein yield between 0.1 and 2 mg/g cells is acceptable. Another concern is protein degradation. Especially with Pichia , protease activity during cell lysis and purification is always an issue. The importance of N-terminal degradation
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