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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
- 保质期:
1 year
- 英文名:
CSF2 / GM-CSF (in E.coli), C-His, recombinant protein
- 库存:
999
- 供应商:
ProteoGenix
- 规格:
50ug/100ug
| 规格: | 50ug | 产品价格: | ¥1856.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥2456.0 |
| Species | Homo sapiens (Human) |
| Isotype | nan |
| Expression system | Prokaryotic expression |
| Buffer | PBS, pH7.5 |
| Size | 100 ug |
| Aliases / Synonyms | nan |
| Delivery condition | Dry Ice |
| Storage condition | 4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection) |
| Kit content | nan |
| Tag | C-terminal His Tag |
| Fragment | Ala18-Glu144 |
| Host Species | Escherichia coli (E.coli) |
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文献和实验.7. Wang, X. et al. 2011. Study of two G-protein coupled receptor variants of human trace amine-associated receptor 5. Sci Rep1: 102 (DOI:10.1038/srep00102).8. Wang, X. and Zhang, S. 2011. Production of a bioengineered G-protein coupled receptor of human
Binding Protein,MBP)的大肠杆菌malE基因,其下游的多克隆位点便于目的基因插入,表达N端带有MBP的融合蛋白。此系统的pMAL载体除了含有氨苄霉素抗性,还含有LacZα基因,目的基因的插入导致其失活,通过X-gal平板可进行蓝白斑筛选。pMAL载体都含有一段编码蛋白酶识别位点的序列,融合蛋白纯化后,通过Factor Xa(X),Enterokinase (E)或 Genenase™ I (G)可将目的蛋白与MBP切割分离。如果不想在切割后留下任何外源氨基酸可以在设计引物时引入XmnⅠ
acids may be precipitated by addition of monovalent cations and two to three volumes of cold 95% ethanol, followed by incubation at 0 to -70 ℃. The DNA or RNA then may be pelleted by centrifugation at 10 to 13,000 x g. for 15 minutes at 4℃. A subsequent
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