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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12 months
- 英文名:
Neuraminidase (Sialidase)
- 供应商:
武汉益普生物科技有限公司
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1280.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥1880.0 |
| 规格: | 1mg | 产品价格: | ¥9776.0 |
| 产品名 | Neuraminidase (Sialidase) |
| 货号 | YXX09401 |
| 别名 | Sialidase, 3.2.1.18, Neuraminidase; nanH |
| 表达系统 | E. coli |
| 种属 | Clostridium perfringens |
| 蛋白长度 | Neuraminidase (Sialidase) is cloned from Clostridium perfringens and expressed in E. coli. |
| 预测分子量 | 44.04 kDa |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | Neuraminidase (Sialidase) has been used to desialylate transferrin in order to study its isoforms in human serum. |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 描述 | Cleaves terminal sialic-acid residues that are α2,3-, α2,6-, or α2,8-linked to Gal, GlcNAc, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids, or glycoproteins. Relative rate of cleavage is α2,3 >α2,8 = α2,6, determined on bonds in tri- and tetrasaccharides. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 说明 | For research use only. |

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文献和实验【信息】Northstar BioProducts(NSBP)
, (Streptococcus sp.), EC 3.2.1.18 1 unit Acetylneuraminyl hydrolase (Sialidase). Sterilized preparation of 120052-1 by membrane filtration Lyophilized powder. Activity: ≥50 units/mg. M.W.: 85,000 (gel filtration) 120055-1 (2-3) Neuraminidase
。在设计实验时,若不使用唾液酸酶(Sialidase)去除这种掩蔽,您所观察到的受体结合能力可能仅仅是冰山一角。 配体特异性的精细差异 Siglec 对唾液酸的识别极其挑剔,不仅区分 α2,3-、α2,6- 连接方式,还高度依赖于深层的聚糖骨架甚至载体蛋白的序列背景。最近的研究证实,硫酸化修饰(如 CHST1 介导的修饰)和特定的 O-聚糖模式是决定 Siglec-3/7/8 等成员结合的关键开关 [15]。CD22 与 Siglec-8 的配体偏好截然不同,仅依赖总唾液酸定量或泛特异性探针,极易
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