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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12 months
- 英文名:
Terminal Transferase (TdT)
- 供应商:
武汉益普生物科技有限公司
- 规格:
500 U/2,500 U
| 规格: | 500 U | 产品价格: | ¥900.0 |
|---|---|---|---|
| 规格: | 2,500 U | 产品价格: | ¥3600.0 |
| 产品名 | Terminal Transferase (TdT) |
| 货号 | YBC05501 |
| 别名 | DNA nucleotidylexotransferase, 2.7.7.31, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, TDT, Terminal transferase, DNTT, TDT |
| 种属 | Bovine |
| 蛋白长度 | Terminal Transferase (TdT) is expressed in E. coli. |
| 预测分子量 | 45 kDa |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | Terminal transferase catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. |
| 状态 | Liquid |
| 保存溶液 | 100mM KAc (pH6.8), 2mM 2-mercaptoethanol, 0.01%Triton X-100 and 50% glycerol. |
| 描述 | Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. It catalyzes the repeated addition of deoxyribonucleotides to oligodeoxyribonucleotides and 3' -Oh of single - and double-stranded DNA. TdT requires an oligodeoxynucleotide of at least three bases as a primer. The addition of Co2+ in the reacton makes tailing more efficient. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 说明 | For research use only. |

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文献和实验Detection of Terminal Transferase in Leukemia
The mere presence of terminal deoxynucleotidyl transferase (TdT), a DNA polymerase, in leukemic cells provides no help in assigning these blast cells to a particular cell lineage (1 ). Differential levels of TdT gene transcription
Terminal Deoxyribonucleotidyl Transferase (EC 2.7.7.31)
Terminal deoxyribonucleotidyl transferase (TdT) (EC 2.7.7.3 1) catalyzes the addition of deoxynucleoside triphosphates to the 3′ ends of oligo-and polynucleotide primers. Different from all the other known DNA polymerases, TdT-catalyzed DNA
Terminal Deoxynucleotidyl Transferase
Terminal Deoxynucleotidyl Transferase (TdT) is an enzyme that catalyzes the repetitive addition of mononucleotides from dNTPs to the terminal 3´-OH of a DNA initiator, accompanied by the release of inorganic phosphate. The enzyme
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