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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgY
- 形态:
Liquid
- 保存条件:
-20°C
- 克隆性:
多克隆
- 标记物:
PerCP
- 保质期:
12 months
- 级别:
一抗
- 供应商:
武汉益普生物科技有限公司
- 宿主:
Chicken
- 应用范围:
FCM, Isotype Control
- 抗体英文名:
Normal Chicken IgY Isotype Control Antibody, PerCP
- 规格:
50T/100T
| 规格: | 50T | 产品价格: | ¥612.0 |
|---|---|---|---|
| 规格: | 100T | 产品价格: | ¥1008.0 |
| 产品名 | Normal Chicken IgY Isotype Control Antibody, PerCP |
| 货号 | FCC09214 |
| 克隆类型 | Polyclonal |
| 同种型 | IgY |
| 宿主 | Chicken |
| 应用 | FCM, Isotype Control |
| 偶联物 | PerCP |
| 内毒素水平 | Please contact with the lab for this information. |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. |
| 稳定性和存储 | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| 说明 | For research use only. |

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文献和实验as many as 6 million dpm to maximize sensitivity. 4) Put the 100,000 dpm samples aside until step #12. 5) After lyophilization, solubilize 200,000 dpm samples in 350 µl IMP buffer. 6) Add 50 µl of antibody or control (normal rabbit serum, mouse isotype control
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
of antibody or control (normal rabbit serum, mouse isotype control,, etc.) to each tube and incubate overnight for 6 h at room temperature. 7) Add 100 µl of Protein-A-Sepharose solution (shake to suspend slurry before pipetting) and incubate on a tube
Protocol for intracytoplasmic staining of cytokines for FACS analysis
for all above cytokine stains. This control negative staining with the actual conjugates is, in our view, much more useful than staining with isotype matched conjugated controls that may have different properties with regard to degree of conjugation, aggregation
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