ZRQSVP5CN默克密理博Millipore Direct

Western Blotting Protocols

cover the surface of the membrane. Incubate for 10 minutes with mild agitation or until signal reaches desired contrast. Rinse the blot with Milli-Q water to stop the reaction. Store the blot out of direct light to minimize fading. Blot

in vivo RNAi Protocols

. no.Q33140) or run on agarose E-gels.   After quantification, use 750 ng of total RNA for first strand synthesis using Superscript® III RT kit and QPCR analysis performed using  SYBR® GreenER™ qPCR Super Mix

【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离

I that is stored at ?80°C (see Note 2). 2. Silicon oil. 3. QIAquick Nucleotide Purification Kit (Qiagen). 4. QIAquick PCR Purification Kit (Qiagen). 5. Splinkerette oligonucleotides (see Table 1 for sequences). Resuspended in TE buffer