尿激酶型纤溶酶原激活因子受体(uPAR)

Recombinant Human PLAUR (uPAR) (carrier-free)

Other Names

CD87, MO3, Plasminogen activator urokinase receptor, uPAR, URKR

Source

Human PLAUR, amino acids (Leu23-Arg303) (Accession# NP_002650), was expressed with a C-terminal His tag in the 293E cell line.

Molecular Mass

The 287 amino acid recombinant protein has a predicted molecular mass of approximately 32.1 kD. The protein migrates at 50 kD in non-reducing and DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Leu.

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Formulation

0.22 µm filtered protein solution is in PBS, pH 7.2.

Endotoxin Level

Less than 1.0 EU per µg of protein as determined by the LAL method.

Concentration

10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial.

Storage & Handling

Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Activity

Biotinylated human PLAUR is able to bind immobilized uPA (2 µg/mL) in a dose dependent manner with a ED₅₀ of < 50 ng/mL. This binding (120 ng/mL of hPLAUR-biotin) can be blocked by non-biotinylated hPLAUR in a dose dependent manner with an IC₅₀ of 150 - 600 ng/mL.

Application

Bioassay

Application Notes

uPA at 2 µg/mL in PBS was immobilized on an ELISA plate and used in the binding assay.

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• Product Details
• Antigen Details
• Documentation
• Reviews
• Related Pages & Pathways
• Related FAQs
• Certificate of Analysis

Pricing & Availability

Regulatory Status

RUO

Other Names

CD87, MO3, Plasminogen activator urokinase receptor, uPAR, URKR

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Biotinylated human PLAUR is able to bind immobilized uPA (2 µg/mL) in a dose dependent manner with a ED₅₀ of < 50 ng/mL (black circle). This binding (120 ng/mL of hPLAUR-biotin) can be blocked by non-biotinylated hPLAUR in a dose dependent manner with an IC₅₀ of 150 - 600 ng/mL (open circle).

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Cat. No.  is scheduled for discontinuation. Please use the availability checker to determine current availability. The 10 µg and 100 µg sizes are available as an alternative.

Description

PLAUR (plasminogen activator urokinase receptor), also known as urokinase plasminogen activator-receptor (uPAR), is part of the plasminogen activation system, which is involved in tissue reorganization, cell migration, cell cycle regulation, and cell adhesion. PLAUR was originally identified as a saturable binding site for urokinase on the cell surface, and thus, restricting plasminogen activation to the immediate vicinity of the cell membrane. Due to lack of transmembrane or cytoplasmic domains, PLAUR may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its C-terminus. A soluble form of PLAUR has been identified. The membrane-bound PLAUR is expressed on granulocytes, monocytes/macrophages, dendritic cells, fibroblasts, endothelial cells, and keratinocytes. This molecule plays a role in cell adhesion and chemotaxis through binding to β1, β2, and β3 integrins. Recently, it was found that soluble PLAUR is increased in plasma of patients with active lupus nephritis, suggesting an association with pathological features of the disease. In addition, soluble PLAUR has been linked as a causative factor and also a biomarker of several diseases including tumor metastasis, focal and segmental glomerulosclerosis, hepatocellular carcinoma, and coronary artery disease.

Product Details

 

Source

Human PLAUR, amino acids (Leu23-Arg303) (Accession# NP_002650), was expressed with a C-terminal His tag in the 293E cell line.

Molecular Mass

The 287 amino acid recombinant protein has a predicted molecular mass of approximately 32.1 kD. The protein migrates at 50 kD in non-reducing and DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Leu.

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Formulation

0.22 µm filtered protein solution is in PBS, pH 7.2.

Endotoxin Level

Less than 1.0 EU per µg of protein as determined by the LAL method.

Concentration

10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Activity

Biotinylated human PLAUR is able to bind immobilized uPA (2 µg/mL) in a dose dependent manner with a ED₅₀ of < 50 ng/mL. This binding (120 ng/mL of hPLAUR-biotin) can be blocked by non-biotinylated hPLAUR in a dose dependent manner with an IC₅₀ of 150 - 600 ng/mL.

Application

Bioassay

Application Notes

uPA at 2 µg/mL in PBS was immobilized on an ELISA plate and used in the binding assay.

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, 

 

Structure

Monomer.

Distribution

Granulocytes, monocytes/macrophages, dendritic cells, fibroblasts, endothelial cells, keratinocytes, and activated T cells.

Function

Convert plasminogen to plasmin, cell adhesion, and chemotaxis.

Ligand/Receptor

uPA; LRP1 (CD91)

Bioactivity

Human PLAUR is able to bind uPA in a dose dependent manner.

Biology Area

Cell Adhesion, Cell Cycle/DNA Replication

Molecular Family

Soluble Receptors