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文献和实验Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
dish (can store for a year). Coat with polyamino acid. Coat coverslips in bulk in 10-15ml 1mg/ml PLL (or 500ug/ml polyornithine), rocking or rotating for a minimum of 30 minutes in a 10 or 15cm tissue culture dish. Save the polyamino acid
Intracellular Cytokine Staining Protocol
anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
sheet of whatman paper (dry as separate coverslips). Keep in a sterile tissue culture dish (can store for a year). Coat with polyamino acid. Coat coverslips in bulk in 10-15ml 1mg/ml PLL (or 500ug/ml polyornithine
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