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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Sorbitol hexaacetate
- 库存:
1
- 供应商:
甄准生物
- 规格:
1G
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文献和实验Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
1 mM EDTA, pH 8.0 10 mM Tris CTAB Buffer 800 mM NaCl 1% (v/v) Sarkosyl 140 mM Sorbitol Autoclave 22 mM EDTA 220 mM Tris, pH 8.0
Electro-Transformation of Yeast电转酵母【Upstate Medical University】
Amberg Lab ,Upstate Medical University1.Grow cells to 1X10E8 or OD600 of 1.2-1.3.2.Spin cells at 5,000rpm for 5 min, and wash pellets in an equal volume of ice cold water.3.Wash in 1/2 volume cold water.4.Wash in 1/25 volume ice-cold 1M. sorbitol
Electro-Transformation of Yeast
Wash in 1/25 volume ice-cold 1M. sorbitol. Treat cells with 25mM. DTT for 10 minutes at room temperature. Wash cells with 1M. cold sorbitol. Resuspend cells in 1/200 original volume cold 1M. sorbitol. Mix 50µl. of cell suspension
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