- ￥6768
- AntibodySystem
- 1.56 - 100 ng/mL
- 0.06 ng/mL
- France
- KAV00801
- 2025年12月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉益普生物科技有限公司
- 检测范围：
1.56 - 100 ng/mL
- 检测方法：
Colorimetric
- 样本：
Plasma, Serum
- 灵敏度：
0.06 ng/mL
- 规格：
96T
| 产品名 | Anti-SARS-CoV-2 Nucleocapsid (NP) Mouse IgG ELISA Kit |
| 货号 | KAV00801 |
| 规格 | 96T |
| 检测方法 | Colorimetric |
| 样本类型 | Plasma, Serum |
| 实验类型 | Quantitative |
| 检测范围 | 1.56 - 100 ng/mL |
| 灵敏度 | 0.06 ng/mL |
| 回收率 | 80-120% |
| 精准度 | CV<15% |
| 描述 | PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant SARS-CoV-2 Nucleocapsid (NP) protein has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-SARS-CoV-2 Nucleocapsid (NP) Mouse IgG present is bound by the immobilized protein. After washing away any unbound substances, a HRP-labeled antibody specific for Mouse IgG is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Mouse SARS-CoV-2 Nucleocapsid (NP) IgG bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| 稳定性和存储 | When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. |
| 保存方法 | 2-8 ℃ |
| 说明 | For Research Use Only. |
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文献和实验OUTLINE This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) (shown here). PROTOCOL
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
at 1:200 dilution) Cy3-conjugated affinity-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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