- ￥6768
- AntibodySystem
- 93.75 - 6,000 ng/mL
- 22.06 ng/mL
- France
- KAV00303
- 2025年12月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉益普生物科技有限公司
- 检测范围：
93.75 - 6,000 ng/mL
- 检测方法：
Colorimetric
- 样本：
Plasma, Serum
- 灵敏度：
22.06 ng/mL
- 规格：
96T
| 产品名 | Anti-SARS-CoV-2 RBD (BA.5) Human IgM ELISA Kit |
| 货号 | KAV00303 |
| 规格 | 96T |
| 应用 | Used for the quantitative determination of Anti-SARS-CoV-2 RBD (BA.5) Human IgM concentration in serum and plasma. |
| 检测方法 | Colorimetric |
| 样本类型 | Plasma, Serum |
| 实验类型 | Quantitative |
| 检测范围 | 93.75 - 6,000 ng/mL |
| 灵敏度 | 22.06 ng/mL |
| 回收率 | 80-120% |
| 精准度 | CV<15% |
| 描述 | PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant SARS-CoV-2 RBD (BA.5) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-SARS-CoV-2 RBD (BA.5) Human IgM present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgM antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-SARS-CoV-2 RBD (BA.5) Human IgM bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| 稳定性和存储 | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition. |
| 保存方法 | 2-8 ℃ |
| 说明 | For Research Use Only. |
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文献和实验-down模式（即间接法）联合抗人检测抗体进行分析，因此不受影响。相比之下，SARS-CoV-2抗原的免疫分析通常使用夹心法模式，容易受到干扰性抗体的影响。 判定干扰性抗体是否是导致错误结果的原因，可以使用市面上商业化的ELISA试剂盒进行检测，这些试剂盒设计用于鉴别嗜异性抗体、HAMA和RF（通常简称为HAMA-ELISA）。鉴定后，常使用HAMA阻断剂来解决干扰问题。该解决方案的缺点是更多的生物活性成分-抗体-被添加到分析中，这可能会导致新的干扰。一般情况，多数生产商会提供几种不同的HAMA阻断
even at titers below 10 ng/ml9,10. The detection limit is of great concern in the context of IgM or IgA assays for active SARS-CoV-2 infections where false negatives are to be avoided at all costs. Especially patients with asymptomatic or mild disease
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
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