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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科技有限公司
- 检测范围:
0.07 - 50 ng/mL
- 检测方法:
Colorimetric
- 样本:
Plasma, Serum
- 灵敏度:
0.05 ng/mL
- 规格:
96T
| 产品名 | Monkeypox Virus (MPXV) B6R Antigen ELISA Kit |
| 货号 | KVV13501 |
| 规格 | 96T |
| 应用 | Used for the quantitative determination of Monkeypox Virus B6R Antigen concentration in serum and plasma. |
| 检测方法 | Colorimetric |
| 样本类型 | Plasma, Serum |
| 实验类型 | Quantitative |
| 检测范围 | 0.07 - 50 ng/mL |
| 灵敏度 | 0.05 ng/mL |
| 回收率 | 80-120% |
| 精准度 | CV<15% |
| 描述 | PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Monkeypox Virus B6R has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Monkeypox Virus B6R Antigen present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for Monkeypox Virus B6R is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Monkeypox Virus B6R Antigen bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| 稳定性和存储 | When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃. |
| 保存方法 | 2-8 ℃ |
| 说明 | For Research Use Only. |

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文献和实验β随着其他因子的影响,其表达水平也会发生相应的变化,一般客户很难察觉,因此这样的恶性造假行为使得消费者被动造假,后果极其严重,因此善意提醒客户警惕此类作假试剂盒!鉴别方法:Ⅰ. 利用干扰实验即可辨别ELISA试剂盒是否检测目的抗原,方法如下:(1)将针对试剂盒特异性的重组蛋白抗原和试剂盒中的检测抗体配置成antibody: antigen≈1:2的混合孵育液。(2)37℃孵育15分钟后,代替原试剂盒中的检测抗体。(3)后续操作按照试剂盒说明书进行,加检测抗体、酶复合物和底物。(4)实验完毕后,检测其标准
Polyclonal Antibody Production 简单快捷制备多抗方法,可用于western、ELISA和免疫/b
Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. -------------------------------------------------------------------------------- Rabbit
corresponds to the dissociation constant Kd. Alternatively, the Kd value can be obtained by fitting the ELISA signal of the individual wells to the equation: Kd = [A][B]/[AB] . ELISA相关仪器试剂设备 酶标仪 洗板机 化学发光检测仪 ELISA检测试剂盒
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