0100-1515安捷伦Bulkhead union, 1/

Isolate It All：siRNA • miRNA • Total RNA • Native Protein

? Recover High Quality Total RNA To assess the quality of total RNA isolated with the mir Vana PARIS Kit, 1 x 106 HeLa cells were homogenized in the Cell Disruption Buffer provided with the kit. Because the homogenization is performed quickly

miRNA microarrays（McManus Lab）(芯片）

or on an Agilent Bioanalyzer. There are two options for small RNA isolation. 1) flashPage, an Ambion product, which is quick but not efficient for running many samples in parallel, or 2) acrylamide gel size fractionation—slow, but many samples can be run

【交流】染色体免疫沉淀技术详细步骤-young lab ！！！

. Resuspend beads every 2 minutes with brief vortexing. 9.  Spin down beads at 16,000 x g for 1 minute at room temperature. 10.  Remove 200 µl of supernatant and transfer to new tube. Reverse crosslink of this IP DNA by incubating at 65°C overnight. 11.