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文献和实验equilibration with low salt buffer for 2 hours to increase recovery.) Load a Luer-lock syringe with 5 ml of low salt buffer and attach to minicolumn. Wash matrix by pushing buffer through matrix at approximately 0.5-1.0 ml/min. II. Binding of DNA
equilibration with low salt buffer for 2 hours to increase recovery.) Load a Luer-lock syringe with 5 ml of low salt buffer and attach to minicolumn. Wash matrix by pushing buffer through matrix at approximately 0.5-1.0 ml/min. II. Binding of DNA to Minicolumn
Applied Separations 2416 6 mL polypropylene cartridge with 20 mm PE frit Disposable 3 way luer adapter Cole Parmer
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