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文献和实验temperature for exactly 5 minutes. Using clean forceps remove the microarray and blow dry with dry nitrogen. Insert the slide into the scanner and scan according the manufacturer’s instruction booklet. For Agilent scanner insert the slide
RNA Isolation From Animal tissue or cell culture
empty, centrifuge the column for 2 min at full speed ($13,000 x g) to completely dry the HiBind® matrix. 11. Elution of RNA. Transfer the column to a clean 1.5 mL microfuge tube (not supplied with kit) and elute the RNA with 15-20 ul of DEPC-treated water
miRNA microarrays(McManus Lab)(芯片)
://www.ambion.com/techlib/prot/bp_12200.pdf) clean-up kit or by ethanol precipitation (http://www.ambion.com/techlib/misc/acetate_precip.pdf) (Note: Do not use a glycogen carrier). or 2) Purifying small RNA on a denaturing acrylimide gel
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