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文献和实验microcentrifuge tubes (VWR) 12 × 32−mm (2‐ml) screw‐cap vials with bonded PTFE/silicon septa (Waters Corporation) Deactivated low
in DMF (v/v) to deprotect the Fmoc group. Agitate for 2 minutes and drain. Repeat with a 12 minute incubation. 4) Rinse the resin by adding 2 mL of DMF, agitating for 15 seconds, and draining. Repeat 3x. 5) Bromoacetylation
Synthesis of a 4‐Selenothymidine Phosphoramidite and Incorporation into Oligonucleotides
of nucleic acids and their protein complexes. Curr. Protoc. Nucleic Acid Chem. 32:1.19.1?1.19.13. © 2008 by John Wiley & Sons, Inc. Keywords: nucleic acid; selenium; derivatization; structure determination; X?ray crystallography
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