- ￥1012.48
- Agilent
- 8003-0619
- 2026年03月01日
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文献和实验150 mM NaCl 15ml 5M 1 mM EDTA 1ml 0.5M 1% Triton X 100 5ml 0.1 % Sodium deoxycholate 0.5g 0.1 % SDS 5ml 10%
In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
tubes (6 mm ID x 50 mm). Washes are done by low-speed spins and all solutions (except fixatives) contain 0.1% Tween 20 to prevent gonads from sticking to glass. The key to making this work consistently is to fix dissected gonads in a combination
Myosin Light Chain Preparation
Buffer A.Homogenizing Buffer: 28 liters: 50 mM KCl,20 mM Tris (pH 8.0),15 mM MCE, 0.2 mM MgAc2 (350 ml 4 M KCl,280 ml 2 M Tris,28 ml 15 M MCE,1.20 g MgAc2) Buffer B.Triton X-100 Buffer: 4 liters of Buffer A plus 40 ml X-100 (1% Triton final) Buffer C
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