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- Agilent
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- 2026年02月28日
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文献和实验Plant DNA methylation analysis by bisulfite genomic sequencing
at sites outside of region intended for amplification to resolve structures like direct or inverted repeats that could interfere with PCR (see comment 2). To a 1.5ml reaction tube, Add genomic DNA dissolved in bidist. water (e.g. 2.5µg
capacity and a loading volume of up to 800 µl. Fused-silica capillary, 100 µm I.D. x 365 µm O.D. (Agilent Technologies or Polymicro Technologies) Fused-silica capillary (size and length depend on the desired split flow rate, see Step 10) Gold
-mediated genome engineering. Cell 154(6):1370-1379. doi: 10.1016/j. cell.2013.08.022 25. DiCarlo JE, Norville JE, Mali P, Rios X, AachJ, Church GM (2013) Genome engineering inSaccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res 41
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