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文献和实验Characterization of Glycosaminoglycans by Capillary Electrophoresis
structure of 1→3, 1→4 linked N -acetyl-d -glucosamine and d -glucuronic acid residues. Hyaluronic acid is an unusual glycosaminoglycan as it is not sulfonated and can have a very high molecular weight, up to 2 � 106 Da compared to 1–3 � 105
capacity and a loading volume of up to 800 µl. Fused-silica capillary, 100 µm I.D. x 365 µm O.D. (Agilent Technologies or Polymicro Technologies) Fused-silica capillary (size and length depend on the desired split flow rate, see Step 10) Gold
Technology (ogt) provides good arrays in 4*44k format compatibel with Agilent scanner and hybridisation equipment [5] Hybridisation buffer materials: 5M Sodium chloride Sigma (S6316) 12x MES prepared as follows: 3.52g MES
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