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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
全基因组的比较基因组杂交技术介绍(Whole-Genome and Custom Fine-Tiling Array CGH)
. Total features 385,000 Feature Size 16um x 16um Array size 17.4mm x 13mm Slide size 1" x 3" (25mm x 75
3. Pellet Cell s in appropriate centrifuge tube 3-5000 x g 5 min (~5000 rpm in a Sorvall SS-34 rotor) 4. Discard supernatant and add 0.4 volume (ie of original volume, here it is 40 ml) TfbI, resupend and ice 15 min. 5. Pellet Cell s
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