5190-8758安捷伦Lead 1000 ug/g Pb

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

of aggregation plate Materials:       Dissecting microscope;       Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3);       1 ml syringe with 26 G 1/2 needle of KSOM medium;       light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);       

Methods for DNA sequencing

in each well is suctioned out with a mouth pipette.Each base-specific sequencing reaction terminated with the short termination mix is loaded using a mouth pipette onto a 0.15 mm X 50 cm X 20 cm, denaturing 5% polyacrylamide gel and electrophoresed for 2.25 hours at 22 mA

Methods for DNA sequencing

with the spin column purification using either the Centri-Sep columns or G-50 microtiter plate procedures given below. 1. Gently tap the column to cause the gel material to settle to the bottom of the column. 2. Remove the column stopper and add 0.75 ml dH