5190-8755安捷伦Iron 5000 ug/g Fe

Methods for the preparation of Fly Extract (FE1 and FE2).

(1500 g) at 4° C for 15 mins. Switch on the 60°C waterbath. After the first spin the extract looks like this: 7. Remove supernatant, including surface fat/oil, from the spun tubes with a pasteur pipette

Methods for DNA sequencing

in each well is suctioned out with a mouth pipette.Each base-specific sequencing reaction terminated with the short termination mix is loaded using a mouth pipette onto a 0.15 mm X 50 cm X 20 cm, denaturing 5% polyacrylamide gel and electrophoresed for 2.25 hours at 22 mA

Methods for DNA sequencing

with the spin column purification using either the Centri-Sep columns or G-50 microtiter plate procedures given below. 1. Gently tap the column to cause the gel material to settle to the bottom of the column. 2. Remove the column stopper and add 0.75 ml dH