5190-7001安捷伦Calibration blank

Quantitating RNA

using a buffered solution with a slightly alkaline pH, such as TE (pH 8.0), as a diluent (and as a blank) to assure accurate and reproducible readings. An example of the variation in A260 /A280 ratio at different pH values is shown in Figure

Chromatin Immunoprecipitation (ChIP)

. 11. Measure the DNA concentration (A 260 ) of the chromatin using a spectrophotometer. Use nuclei lysis buffer for ChIP devoid of SDS as a blank. Typically, the chromatin concentration should be >750 ng/µL. The A260 / A280 ratio