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The Basics: RNase Control

can be used as a convenient, noncarcinogenic alternative to DEPC for treating small volumes of precious reagents and solutions such as Tris and MOPS that cannot be DEPC treated. The reagent is supplied as a 25X stock. After the RNAsecure stock is diluted

ChIP-Chip E. coli

°C for 2 nights and one day (about 36 hours) in a light tight container, ideally in a hybridisation oven with a rotisserie. Fit the slides vertically and rotate the chambers at a speed at 4 rpm (setting 10 for Agilent hybridisation oven).

Chromatin Immunoprecipitation (ChIP)

, for aspiration; see Step 20) Ice Incubators preset to 55°C, 65°C Microcentrifuge Micropipettor with tips PCR apparatus Rotator Sonicator Spectrophotometer Spin column (QIAGEN) Tubes (conical, 15-mL) Tubes