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文献和实验General Principles of Immunoprecipitation
with either phosphate or Tris,depending on your needs.NP40 buffer is:1% NP-40 or Triton X-1000.15 molar NaCl0.01 molar sodium phosphate,pH 7.21% Trasylol,a 1:100 dilution of what comes from MobayIf Tris is substituted for phosphate,use 50 mmolar Tris-HCl,pH 7.2 .Add
A PC-based program for evaluation of Comparative Genomic Hybridization (CGH) experiments
. The maximum image size is 1320x1035x12 bit. The 100 W mercury lamp and the diaphragms of the microscope were precisely adjusted to get a homogeneous illumination of the optical field. For each metaphase spread three gray-level images were digitized
Hints and precautions for the care, feeding and breeding of Neurospora
. For most manipulations and observations (ascospore isolations; inspection of spores, perithecia, etc.), an excellent general-purpose microscope is the Stereo Zoom 7 with base, Bausch & Lomb Cat. No. BVIO70 with 10X oculars (1984 price $1417). This provides 10X-70X
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