- ￥5133.59
- Agilent
- 5043-9319
- 2025年10月13日
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文献和实验. First, the pre-crRNA array and tracrRNA are transcribed from theCRISPR locus. Second, tracrRNA hybridizes to the direct repeats of pre-crRNAand associates with Cas9 as a duplex, which mediates the processing of thepre-crRNA into mature crRNAs containing
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to quench formaldehyde. 4. Rinse cells twice with 5 ml 1x PBS. Harvest cells using silicon scraper. 5. Pool cells in 50 ml conical tubes and spin at 1,350 x g for 5 minutes at 4°C (Sorvall Legend RT centrifuge with swinging bucket rotor). Discard
in water gradient, both containing 0.1% (v/v) TFA, at 1 mL/min over 30 minutes at 60 °C with a C18, 5 μm, 50 X 2 mm column) and MALDI (Fig. 5). Purification with reverse phase HPLC on a Vydac C18 column (10 μm, 22 mm x 250 mm) proceeded, using
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