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文献和实验miRNA microarrays(McManus Lab)(芯片)
under desiccation and vacuum. Do not use if exposed to moisture, avoid using clumps. h) IMMEDIATELY after succinic anhydride dissolves, mix in 15 ml of 1M sodium borate pH 8.0. i) Quickly pour the buffered blocking solution into a clean, dry glass slide
using a buffered solution with a slightly alkaline pH, such as TE (pH 8.0), as a diluent (and as a blank) to assure accurate and reproducible readings. An example of the variation in A260 /A280 ratio at different pH values is shown in Figure
Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer
) Micro‐90 cleaning solution (Cole‐Parmer) 10× bromphenol blue/xylene cyanol loading dye (see recipe )
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