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文献和实验Extraction and PCR Analyis of DNA from Mice Tails
Place numbered Eppendorf tubes in dry ice. Cut 0.5cm from end of tails and place in tubes.Store at -80℃ until analyzed. Add 0.3ml of STE/PK solution to the Eppendorf tubes. Incubate at 55℃ overnight with rocking or for several hours
150 mM NaCl 15ml 5M 1 mM EDTA 1ml 0.5M 1% Triton X 100 5ml 0.1 % Sodium deoxycholate 0.5g 0.1 % SDS 5ml 10%
until the others are ready. 3. Transfer to a 50 ml conical tube and centrifuge for 10 min at 2000 x g at 4 C. 4. Resuspend each sample in 1 ml of 10 mM sodium azide and place on ice. 5. Calculate the volume of resuspended cells that would translate to an OD600 nm
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