14256021安捷伦HF Mega BE-SAX, 2gm

Large Scale Plasmid Preps: Qiagen/Cesium Method

according to the Qiagen Maxi or Mega prep protocol. Resuspend the DNA in 3.7 mL of T.E. Add 3.7 gm. CsCl and 0.37 mL of 1 mg/mL ethidium bromide. Check the density of the DNA solusion by placing 0.01 mL (= V) on an analytical balance and recording

张峰手稿：基因工程技术之CRISPR-Cas9

):819–823. doi: 10.1126/science. 1231143 20. Mali P, Yang L, Esvelt KM, Aach J, Guell M,DiCarlo JE, Norville JE, Church GM (2013)RNA-guided human genome engineering via Cas9. Science 339(6121):823–826. doi: 10. 1126/science.1232033 21. Jinek M

DNA疫苗实验方法

you want). See "Plasmid DNA Preparation" protocols for detailed information. Each TA muscle is injected with 50 µl of DNA solution. To inject plasmid DNA use a 27GX3/4" (0.4x20mm) needle attached to a 1 ml tuberculin syringe. A piece of polyethylene