14102032安捷伦HF Bond Elut-PH, 50

Proteasome Preparation from Human Brain

with water. Make up the Homogenising Buffer (3ml/g tissue) in the following way:- Tris 500mM = 30.3g/500ml. Adjust to pH7.5 with HCl. ATP 100mM = 0.551g/10ml. Adjust to pH7.5 with NaOH (can be stored at -20°C). MgCl2 50mM = 1.02g/100ml. DTT

ChIP-Chip E. coli

mg/ml Proteinase K in TBS (store up to 1 year at -20 C) 1 ml 100mM PMSF or 0.5 ml 250 mM Pefabloc   Final concentration For 50ml Lysis Buffer 10mM Tris (pH 8.0) 500μL 1

种子蛋白质系统分析：（SDS-聚丙烯酰胺凝胶电泳法）

ml，溴酚蓝0.5g，SDS l0g，溶于水，用HCI调PH至8.0，定容至500ml。( 11)脱色液 甲醇：乙酸：水＝5：1 ：5（体积比）( 12)染色液（0.25%考马斯亮蓝R250）1.00g考马斯亮蓝R250，用脱色液400ml溶解，过滤备用。( 13)低分子质量标准蛋白溶液，包括溶菌酶（MW=14400Da）大豆胰蛋白酶抑制剂（215000）、碳酸酐酶（310000）、卵白蛋白（450000）、牛血清白蛋白 (662000）、磷酸化酶B (925000）。用样品缓冲液配成2mg/ml