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- Agilent
- 12272011
- 2025年07月05日
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文献和实验Chromatin Interaction Analysis Using Paired‐End Tag Sequencing
(see recipe ) 20% (v/v) Triton X‐100 25:24:1 phenol/chloroform/isoamyl alcohol, pH 7.9 (Ambion
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For 2000 ml 0.06x 20x SSPE 6.0 ml ddH2O 1994.0 ml 2000.0 ml Wash III (Purchase 1x solution from Agilent) Appendix: Oligos for blunt end ligation: oJW102: 5’-GCGGTGACCCGGGAGATCTGAATTC oJW103: 5’-GAATTCAGATC Preparation of 15 µM
is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea
, I figured out that I had very exaggerated A260 readings due to phenol contamination in my RNA. So when I thought I had 45 � of RNA, I really only had maybe..10? In addition, phenol inhibits PCR. In a 2002 publication by Agilent, they found that 0.5% (w/v
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